Matrix metalloproteinase-10 regulates stemness of ovarian cancer stem-like cells by activation of canonical Wnt signaling and can be a target of chemotherapy-resistant ovarian cancer

Oncotarget. 2016 May 3;7(18):26806-22. doi: 10.18632/oncotarget.8645.

Abstract

Epithelial ovarian cancer (EOC) is one of the most lethal cancers in females. Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) have been reported to be origin of primary and recurrent cancers and to be resistant to several treatments. In this study, we identified matrix metalloproteinase-10 (MMP10) is expressed in CSCs/CICs of EOC. An immunohistochemical study revealed that a high expression level of MMP10 is a marker for poor prognosis and platinum resistance in multivariate analysis. MMP10 gene overexpression experiments and MMP10 gene knockdown experiments using siRNAs revealed that MMP10 has a role in the maintenance of CSCs/CICs in EOC and resistance to platinum reagent. Furthermore, MMP10 activate canonical Wnt signaling by inhibiting noncanonical Wnt signaling ligand Wnt5a. Therefore, MMP10 is a novel marker for CSCs/CICs in EOC and that targeting MMP10 is a novel promising approach for chemotherapy-resistant CSCs/CICs in EOC.

Keywords: MMP10; cancer stem cell; chemoresistance; ovarian cancer.

MeSH terms

  • Animals
  • Biomarkers, Tumor / analysis
  • Carcinoma, Ovarian Epithelial
  • Drug Resistance, Neoplasm / physiology*
  • Enzyme Activation / physiology
  • Female
  • Gene Expression Regulation, Neoplastic / physiology
  • Heterografts
  • Humans
  • Kaplan-Meier Estimate
  • Matrix Metalloproteinase 10 / metabolism*
  • Mice
  • Mice, Nude
  • Neoplasms, Glandular and Epithelial / metabolism
  • Neoplasms, Glandular and Epithelial / mortality
  • Neoplasms, Glandular and Epithelial / pathology*
  • Neoplastic Stem Cells / metabolism
  • Neoplastic Stem Cells / pathology*
  • Ovarian Neoplasms / metabolism
  • Ovarian Neoplasms / mortality
  • Ovarian Neoplasms / pathology*
  • Proportional Hazards Models
  • Wnt Signaling Pathway / physiology*

Substances

  • Biomarkers, Tumor
  • MMP10 protein, human
  • Matrix Metalloproteinase 10