Inducible targeting of cDCs and their subsets in vivo

J Immunol Methods. 2016 Jul:434:32-8. doi: 10.1016/j.jim.2016.04.004. Epub 2016 Apr 9.

Abstract

Conventional dendritic cells (cDCs) are essential immune cells linking the innate and adaptive immune system. cDC depletion in mice is an important method to study the function of these cells in vivo. Here we report an inducible in vivo system for cDC depletion in which excision of a loxP flanked Stop signal enables expression of the human diphtheria toxin receptor (DTR) under the control of Zbtb46 (zDC(lSlDTR)). cDCs can be specifically depleted by combining zDC(lSlDTR) mice with a Csf1r(Cre) driver line. In addition, we show that zDC(Cre) mice can be used to produce cDC specific conditional knockout mice (Irf8, Irf4, Notch2) which lack specific subsets of cDCs.

Keywords: Dendritic cells; In vivo depletion models; zDC.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / cytology*
  • Bone Marrow Cells / immunology
  • Crosses, Genetic
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Flow Cytometry / methods
  • Heparin-binding EGF-like Growth Factor / immunology*
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Spleen / immunology

Substances

  • Heparin-binding EGF-like Growth Factor