Abstract
Shigellosis remains a serious issue throughout the developing countries, particularly in children under the age of 5. Numerous strategies have been tested to develop vaccines targeting shigellosis; unfortunately despite several years of extensive research, no safe, effective, and inexpensive vaccine against shigellosis is available so far. Here, we illustrate in detail an approach to identify and establish immunogenic outer membrane proteins from Shigella flexneri 2a as subunit vaccine candidates.
Keywords:
B cell; Macrophages; Outer membrane proteins; Protective immune response; Shigella flexneri 2a; T cell.
MeSH terms
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Animals
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Antigens, Bacterial / immunology
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Outer Membrane Proteins / immunology
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Bacterial Outer Membrane Proteins / isolation & purification
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Cloning, Molecular
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Female
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Gene Expression
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Lymphocyte Activation / immunology
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Macrophages, Peritoneal / cytology
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Macrophages, Peritoneal / immunology
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Male
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Mice
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Recombinant Proteins / isolation & purification
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Sequence Analysis
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Shigella / immunology*
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Shigella Vaccines / genetics
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Shigella Vaccines / immunology*
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Shigella Vaccines / isolation & purification
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology
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Vaccines, Subunit / genetics
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Vaccines, Subunit / immunology
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Vaccines, Subunit / isolation & purification
Substances
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Recombinant Proteins
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Shigella Vaccines
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Vaccines, Subunit