Longitudinal assessment of subcortical gray matter volume, cortical thickness, and white matter integrity in HIV-positive patients

J Magn Reson Imaging. 2016 Nov;44(5):1262-1269. doi: 10.1002/jmri.25263. Epub 2016 Apr 15.

Abstract

Purpose: To longitudinally evaluate the cortical thickness and deep gray matter structures volume, measured from T1 three-dimensional (3D) Gradient echo-weighted imaging, and white matter integrity, assessed from diffusion tensor imaging (DTI) of HIV-positive patients.

Materials and methods: Twenty-one HIV-positive patients on stable highly active antiretroviral therapy (HAART) with CD4+ T lymphocytes count >200 cells/mL and viral load <50 copies/mL underwent two magnetic resonance imaging (MRI) scans with a median interval of 26.6 months. None of the patients had HIV-related dementia. T1 3D magnetization prepared rapid gradient echo-weighted imaging and DTI along 30 noncolinear directions were performed using a 1.5 Tesla MR scanner. FreeSurfer was used to perform cortical volumetric reconstruction and segmentation of deep gray matter structures. For tract-based spatial statistics analysis, a white matter skeleton was created, and a permutation-based inference with 5000 permutations, with a threshold of P < 0.05 was used to identify abnormalities in fractional anisotropy (FA). The median, radial, and axial diffusivities were also projected onto the mean FA skeleton.

Results: There were no significant differences in cortical thickness, deep gray matter structures volumes or diffusivity parameters between scans at the two time points (considering P < 0.05).

Conclusion: No longitudinal differences in cortical thickness, deep gray matter volumes, or white matter integrity were observed in an HIV-positive population on stable HAART, with undetectable viral load and high CD4+ T lymphocytes count. J. Magn. Reson. Imaging 2016;44:1262-1269.

Keywords: HIV; HIV-associated neurocognitive disorders; cortical thickness; diffusion tensor imaging; longitudinal follow-up.

Publication types

  • Clinical Trial

MeSH terms

  • Adult
  • Antiretroviral Therapy, Highly Active / methods
  • Diffusion Tensor Imaging / methods*
  • Encephalitis, Viral / drug therapy*
  • Encephalitis, Viral / immunology
  • Encephalitis, Viral / pathology*
  • Female
  • Gray Matter / immunology
  • Gray Matter / pathology*
  • HIV Infections / drug therapy*
  • HIV Infections / immunology
  • HIV Infections / pathology*
  • Humans
  • Longitudinal Studies
  • Male
  • Middle Aged
  • Organ Size / immunology
  • Treatment Outcome
  • Viral Load / immunology
  • White Matter / immunology
  • White Matter / pathology*