Genome characterization of feline morbillivirus from Italy

Maurilia Marcacci; Eliana De Luca; Guendalina Zaccaria; Morena Di Tommaso; Iolanda Mangone; Giovanni Aste; Giovanni Savini; Andrea Boari; Alessio Lorusso

doi:10.1016/j.jviromet.2016.05.002

Genome characterization of feline morbillivirus from Italy

J Virol Methods. 2016 Aug:234:160-3. doi: 10.1016/j.jviromet.2016.05.002. Epub 2016 May 4.

Authors

Maurilia Marcacci¹, Eliana De Luca¹, Guendalina Zaccaria¹, Morena Di Tommaso², Iolanda Mangone¹, Giovanni Aste², Giovanni Savini¹, Andrea Boari², Alessio Lorusso³

Affiliations

¹ Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
² Faculty of Veterinary Medicine, University of Teramo, Italy.
³ Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy. Electronic address: [email protected].

Abstract

Feline morbillivirus (FeMV) has been recently identified by RT-PCR in the urine sample of a nephropathic cat in Italy. In this report, we describe the whole genome sequence of strain Piuma/2015 obtained by combination of sequence independent single primer amplification method (SISPA) and next generation sequencing (NGS) starting from RNA purified from the infected urine sample. The existence in Germany and Turkey of FeMVs from cats divergent from Piuma/2015, suggests the presence of FeMV heterogeneity in Europe as it has been described previously in Japan and China.

Keywords: Cat; Feline morbillivirus; Italy; NGS; SISPA; Urine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cat Diseases / virology
Cats
DNA Primers
Genome, Viral*
High-Throughput Nucleotide Sequencing
Italy
Morbillivirus / genetics*
Morbillivirus / isolation & purification
Morbillivirus Infections / veterinary
Morbillivirus Infections / virology
Nucleic Acid Amplification Techniques*
Phylogeny
RNA, Viral / genetics
Urine / virology

Substances

DNA Primers
RNA, Viral