The CSF IL-10 concentration is an effective diagnostic marker in immunocompetent primary CNS lymphoma and a potential prognostic biomarker in treatment-responsive patients

Eur J Cancer. 2016 Jul:61:69-76. doi: 10.1016/j.ejca.2016.03.080. Epub 2016 May 5.

Abstract

Introduction: We aimed to confirm the diagnostic value and to evaluate the pre- and post-therapeutic prognostic value of cerebrospinal fluid (CSF) concentrations of interleukin (IL)-10 and IL-6 in patients with diffuse large B-cell primary central nervous system lymphoma (PCNSL).

Patients and methods: IL-10 and IL-6 concentrations were measured in 79 patients with PCNSL at diagnosis and in 40 control individuals. Fifty-four PCNSL patients underwent repeat assessments starting at diagnosis.

Results: The IL-10 concentration distinguished PCNSL from other neurologic diseases with a sensitivity of 88.6% and a specificity of 88.9% with a cutoff of 4 pg/ml. In a multivariate analysis of PCNSL patients, CSF involvement was associated with a higher IL-10 concentration (mean log (IL-10) of 4.4 versus 2.5 pg/ml, respectively, p = 0.0004). The pre-therapeutic IL-10 concentration had no prognostic impact on outcome. The IL-10 concentration decreased after treatment for most patients tested. Among patients with complete remission or partial remission, as evaluated by magnetic resonance imaging (MRI), a persistent detectable IL-10 level in the CSF at the end of treatment was associated with a negative impact on progression-free survival (PFS) (1-year PFS: 15%, 95% confidence interval [CI]: 2.5-38% versus 59%, 95% CI: 32-78%, respectively, p = 0.0004).

Conclusion: Our study confirmed that IL-10 is a useful biomarker for the diagnosis of PCNSL. We highlight new findings showing that the IL-10 level in the CSF could be used as a surrogate marker for CSF involvement and that the post-treatment IL-10 concentration could complement standard MRI for therapeutic response assessment in PCNSL.

Keywords: Biomarker; Cerebrospinal fluid; IL-10; IL-10:IL-6 ratio; Primary CNS lymphoma; Response assessment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / cerebrospinal fluid*
  • Case-Control Studies
  • Central Nervous System Neoplasms / cerebrospinal fluid
  • Central Nervous System Neoplasms / diagnosis*
  • Disease-Free Survival
  • Female
  • Humans
  • Interleukin-10 / cerebrospinal fluid*
  • Interleukin-6 / cerebrospinal fluid
  • Logistic Models
  • Lymphoma, Non-Hodgkin
  • Male
  • Middle Aged
  • Prognosis
  • Sensitivity and Specificity
  • Young Adult

Substances

  • Biomarkers, Tumor
  • IL10 protein, human
  • IL6 protein, human
  • Interleukin-6
  • Interleukin-10