Detection of 1,N(2)-propano-2'-deoxyguanosine in human urine by stable isotope dilution UHPLC-MS/MS analysis

J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Jun 15:1023-1024:68-71. doi: 10.1016/j.jchromb.2016.04.029. Epub 2016 Apr 20.

Abstract

A sensitive and accurate stable isotope dilution UHPLC-MS/MS method was developed and validated for the detection and quantification of ProdG adducts in human urine, a surrogate for the ProdG adducts in genomic DNA of human. A specific solid phase extraction (SPE) approach was established for selective enrichment of urinary ProdG adducts and elimination of urinary matrix facilitating the coupled MS/MS detection. The recovery of the method is estimated about 84.8-107.2%, and the precision are about 0.8-3.6% for intraday and 2.8-10.0% for interday. Due to that the matrix effect is efficiently eliminated by SPE pretreatment, the limits of detection (LODs, S/N=3) and quantification (LOQs, S/N=10) are decreased to 100 and 300 amol for urinary ProdG adducts, respectively. By coupling the developed SPE pretreatment with the UHPLC-MS/MS analysis, ProdG adducts were accurately quantified in healthy human urine.

Keywords: 1,N(2)-propano-2′-deoxyguanosine adducts; Human urine; SPE; UHPLC–MS/MS.

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • DNA / chemistry
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / chemistry
  • Deoxyguanosine / urine
  • Female
  • Humans
  • Isotopes
  • Limit of Detection
  • Linear Models
  • Male
  • Reproducibility of Results
  • Solid Phase Extraction
  • Tandem Mass Spectrometry / methods*

Substances

  • Isotopes
  • 1,N(2)-propanodeoxyguanosine
  • DNA
  • Deoxyguanosine