Trichloroethylene exposure aggravates behavioral abnormalities in mice that are deficient in superoxide dismutase

Regul Toxicol Pharmacol. 2016 Aug:79:83-90. doi: 10.1016/j.yrtph.2016.05.007. Epub 2016 May 7.

Abstract

Trichloroethylene (TCE) has been implicated as a causative agent for Parkinson's disease (PD). The administration of TCE to rodents induces neurotoxicity associated with dopaminergic neuron death, and evidence suggests that oxidative stress as a major player in the progression of PD. Here we report on TCE-induced behavioral abnormality in mice that are deficient in superoxide dismutase 1 (SOD1). Wild-type (WT) and SOD1-deficient (Sod1(-/-)) mice were intraperitoneally administered TCE (500 mg/kg) over a period of 4 weeks. Although the TCE-administrated Sod1(-/-) mice showed marked abnormal motor behavior, no significant differences were observed among the experimental groups by biochemical and histopathological analyses. However, treating mouse neuroblastoma-derived NB2a cells with TCE resulted in the down regulation of the SOD1 protein and elevated oxidative stress under conditions where SOD1 production was suppressed. Taken together, these data indicate that SOD1 plays a pivotal role in protecting motor neuron function against TCE toxicity.

Keywords: Neurotoxicity; Oxidative stress; Parkinson’s disease; Superoxide dismutase; Trichloroethylene.

MeSH terms

  • Animals
  • Behavior, Animal / drug effects*
  • Brain / drug effects*
  • Brain / enzymology
  • Brain / pathology
  • Brain / physiopathology
  • Cell Line, Tumor
  • Dopaminergic Neurons / drug effects
  • Dopaminergic Neurons / enzymology
  • Dopaminergic Neurons / pathology
  • Genotype
  • Mice, Knockout
  • Motor Activity / drug effects*
  • Neuroblastoma / enzymology
  • Neuroblastoma / pathology
  • Neurotoxicity Syndromes / enzymology
  • Neurotoxicity Syndromes / etiology*
  • Neurotoxicity Syndromes / genetics
  • Neurotoxicity Syndromes / physiopathology
  • Oxidative Stress / drug effects
  • Phenotype
  • Rotarod Performance Test
  • Superoxide Dismutase-1 / deficiency*
  • Superoxide Dismutase-1 / genetics
  • Time Factors
  • Trichloroethylene / toxicity*

Substances

  • Trichloroethylene
  • Sod1 protein, mouse
  • Superoxide Dismutase-1