Clinical manifestations of Zika virus, chikungunya virus, and dengue virus infections can be similar. To improve virus detection, streamline molecular workflow, and decrease test costs, we developed and evaluated a multiplex real-time reverse transcription PCR for these viruses.
Keywords: Zika virus; arboviruses; chikungunya virus; dengue virus; multiplex molecular diagnostics; real-time RT-PCR; vector-borne infections; viruses.