Ascitic Calprotectin is a Novel and Accurate Marker for Spontaneous Bacterial Peritonitis

J Clin Lab Anal. 2016 Nov;30(6):1139-1145. doi: 10.1002/jcla.21994. Epub 2016 May 17.

Abstract

Background: Spontaneous bacterial peritonitis (SBP) is a known complication of advanced cirrhosis and presents a high mortality rate. A polymorphonuclear (PMN) cell count >250/μl in the ascitic fluid is the current gold standard for diagnosing SBP.

Aim: We evaluated the accuracy of a point-of-care test (POCT) for ascitic calprotectin in diagnosing patients with SBP.

Methods: Eighty-eight patients admitted with decompensation of liver cirrhosis were studied including 41 patients (46.6%) with SBP. Ascitic calprotectin was measured using a quantitative POCT developed by Bühlmann® .

Results: Calprotectin levels correlated with PMN cell count and other inflammatory markers and were significantly higher in patients with SBP. An optimal cutoff of calprotectin above 1.57 μg/ml presented high sensitivity (87.8%), specificity (97.9%), and positive (97.3%) and negative (90.2%) predictive values for diagnosing SBP. Using calprotectin selectively in patients with a serum albumin-ascites gradient above 11 g/l further increased the sensitivity and negative predictive values of the test.

Conclusion: Ascitic calprotectin appears to be a reliable method for diagnosing SBP in patients with liver cirrhosis. It may present an alternative to other conventional diagnostic methods.

Keywords: ascites; liver cirrhosis; spontaneous bacterial peritonitis.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Ascitic Fluid / metabolism*
  • Bacterial Infections / complications*
  • Biomarkers
  • Female
  • Humans
  • Leukocyte L1 Antigen Complex / metabolism*
  • Liver Cirrhosis / complications
  • Male
  • Middle Aged
  • Peritonitis / etiology*
  • Peritonitis / microbiology*
  • Prospective Studies
  • ROC Curve
  • Statistics, Nonparametric

Substances

  • Biomarkers
  • Leukocyte L1 Antigen Complex