Abstract
In humans, two endothelin receptors, ETa and ETb, are activated by three endogenous 21-mer cyclic peptides, ET-1, ET-2, and ET-3, which control various physiological processes, including vasoconstriction, vasodilation, and stimulation of cell proliferation. The first stage of this study it to produce a stable solubilized and purified receptor in a monodisperse state. This article is focused on the engineering, expression, purification, and characterization of the endothelin receptor B for subsequent structural and functional studies.
MeSH terms
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Animals
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Baculoviridae / genetics
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Biphenyl Compounds / chemistry
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Blotting, Western
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Dipeptides / chemistry
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Endothelin Receptor Antagonists / chemistry
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Endothelins / chemistry
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Genetic Engineering / methods
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Humans
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Peptide Fragments / chemistry
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Protein Denaturation
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Protein Stability
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Receptor, Endothelin B / chemistry*
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Receptor, Endothelin B / genetics
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Receptor, Endothelin B / isolation & purification*
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Receptor, Endothelin B / metabolism
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Sf9 Cells
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Temperature
Substances
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Biphenyl Compounds
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Dipeptides
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EDNRB protein, human
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Endothelin Receptor Antagonists
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Endothelins
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IFITM5 protein, human
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IRL 2500
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Membrane Proteins
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Peptide Fragments
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Receptor, Endothelin B
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Recombinant Fusion Proteins
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sovateltide