Effects of short-term hypoxia and seawater acidification on hemocyte responses of the mussel Mytilus coruscus

Yanming Sui; Hui Kong; Yueyong Shang; Xizhi Huang; FangLi Wu; Menghong Hu; Daohui Lin; Weiqun Lu; Youji Wang

doi:10.1016/j.marpolbul.2016.05.001

Effects of short-term hypoxia and seawater acidification on hemocyte responses of the mussel Mytilus coruscus

Mar Pollut Bull. 2016 Jul 15;108(1-2):46-52. doi: 10.1016/j.marpolbul.2016.05.001. Epub 2016 May 18.

Authors

Yanming Sui¹, Hui Kong², Yueyong Shang³, Xizhi Huang³, FangLi Wu³, Menghong Hu⁴, Daohui Lin⁵, Weiqun Lu⁶, Youji Wang⁷

Affiliations

¹ College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou 310058, China. Electronic address: [email protected].
² College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou 310058, China.
³ College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
⁴ College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Department of Integrative Ecophysiology, Alfred-Wegener-Institute Helmholtz Center for Polar- and Marine Research, 27570 Bremerhaven, Germany.
⁵ Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou 310058, China; Department of Environmental Science, Zhejiang University, Hangzhou 310058, China.
⁶ College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China. Electronic address: [email protected].
⁷ College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Department of Integrative Ecophysiology, Alfred-Wegener-Institute Helmholtz Center for Polar- and Marine Research, 27570 Bremerhaven, Germany; Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Zhejiang University, Hangzhou 310058, China. Electronic address: [email protected].

PMID: 27207025
DOI: 10.1016/j.marpolbul.2016.05.001

Abstract

Hypoxia often intensifies with rising dissolved CO2, but the concurrent effects of hypoxia and acidification on bivalves are largely unknown. In this study, immune responses of hemocytes in the mussel Mytilus coruscus were examined under six combinations of pH (7.3, 7.7 and 8.1) and dissolved oxygen (DO) concentrations (2mgL(-1), 6mgL(-1)) for 72h. Generally, total hemocyte account, phagocytosis, esterase and lysosomal content were reduced under low DO and pH conditions, whereas hemocyte mortality and reactive oxygen species production increased under low DO and pH. Both hypoxia and low pH have negative effects on mussels, but the effects of pH are not as strong as DO. Moreover, significant interactions between DO and pH occurred. However, acidification generally doesn't aggravate the effects induced by hypoxia. Acidification and hypoxia may increase disease risk and impact the aquaculture of this species.

Keywords: Acidification; Flow cytometry; Hemocyte; Hypoxia; Immune response; Mytilus coruscus.

MeSH terms

Animals
Hemocytes / drug effects*
Hydrogen-Ion Concentration
Hypoxia*
Mytilus / drug effects*
Seawater / chemistry*