Inhibition of human norovirus by a viral polymerase inhibitor in the B cell culture system and in the mouse model

Antiviral Res. 2016 Aug:132:46-9. doi: 10.1016/j.antiviral.2016.05.011. Epub 2016 May 17.

Abstract

The recently developed human norovirus (HuNoV) B cell culture and mouse models hold promise for drug discovery and development but their suitability for antiviral studies has not been assessed. We demonstrate the inhibitory effect of the nucleoside analogue 2'-C-methylcytidine (2CMC) on HuNoV replication in the human B cell BJAB cell line and in Balb/c Rag/gamma chain-deficient (Rag-γc(-/-)) mice. These data suggest the applicability of both models for future study and development of antiviral drugs for the treatment of HuNoV infections.

Keywords: Antiviral; Enteric virus; Human norovirus; Polymerase inhibitor; Positive-sense RNA virus.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / pharmacology*
  • B-Lymphocytes / drug effects*
  • B-Lymphocytes / virology*
  • Caliciviridae Infections / drug therapy
  • Caliciviridae Infections / virology*
  • Cell Line
  • Disease Models, Animal
  • Humans
  • Mice
  • Mice, Knockout
  • Norovirus / drug effects*
  • Norovirus / physiology*
  • Virus Replication / drug effects

Substances

  • Antiviral Agents