Complete Nucleotide Sequence of an IncI2 Plasmid Coharboring blaCTX-M-55 and mcr-1

Antimicrob Agents Chemother. 2016 Jul 22;60(8):5014-7. doi: 10.1128/AAC.00774-16. Print 2016 Aug.

Abstract

We report the complete nucleotide sequence of a plasmid, pA31-12, carrying blaCTX-M-55 and mcr-1 from a chicken Escherichia coli isolate. pA31-12 has an IncI2 replicon that displays extensive sequence similarity with pHN1122-1-borne blaCTX-M-55 and pHNSHP45-borne mcr-1 Insertion sequences ISEcp1 and ISApl1 are responsible for the mobilization of blaCTX-M-55 and mcr-1, respectively. The colocalization of mcr-1 with an extended-spectrum β-lactamase gene on a conjugative plasmid may accelerate the dissemination of both genes by coselection.

MeSH terms

  • Base Sequence / genetics*
  • Escherichia coli / genetics*
  • Escherichia coli Proteins / genetics*
  • Plasmids / genetics*
  • beta-Lactamases / genetics*

Substances

  • Escherichia coli Proteins
  • MCR-1 protein, E coli
  • beta-Lactamases