The cyclic AMP assay is a functional assay that is commonly used to determine the pharmacological behavior (agonists, antagonists, inverse agonists) of G-protein-coupled receptor (GPCR) ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available non-radioligand ready-to-use kits and Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB2 receptor.
Keywords: 3-Isobutyl-1-methylxanthine (IBMX); Agonist; Antagonist; Cannabinoid receptor type 2; Cyclic AMP; Forskolin (FSK); Inverse agonist.