Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells

S F Tsai; D I Martin; L I Zon; A D D'Andrea; G G Wong; S H Orkin

doi:10.1038/339446a0

Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells

Nature. 1989 Jun 8;339(6224):446-51. doi: 10.1038/339446a0.

Authors

S F Tsai¹, D I Martin, L I Zon, A D D'Andrea, G G Wong, S H Orkin

Affiliation

¹ Division of Hematology-Oncology, Children's Hospital, Boston, Massachusetts.

PMID: 2725678
DOI: 10.1038/339446a0

Abstract

Genes expressed in erythroid cells contain binding sites for a cell-specific factor believed to be an important regulator for this haematopoietic lineage. Using high-level transient expression in mammalian cells, we have identified complementary DNA encoding the murine protein. The factor, a new member of the zinc-finger family of DNA-binding proteins, is restricted to erythroid cells at the level of RNA expression and is closely homologous between mouse and man.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA / genetics*
DNA-Binding Proteins / blood
DNA-Binding Proteins / genetics*
Erythrocytes / metabolism*
Erythroid-Specific DNA-Binding Factors
Genes*
Humans
Mice
Molecular Sequence Data
RNA, Messenger / genetics
Transcription Factors*
Transcription, Genetic

Substances

DNA-Binding Proteins
Erythroid-Specific DNA-Binding Factors
RNA, Messenger
Transcription Factors
DNA