Comparison of SPE, IFE, and FLC in Monitoring Patients with Multiple Myeloma After Autologous Stem Cell Transplantation

Wei Li; Jia-Zi Zhou; Hui-Rong Chang; Li-Jun Dai; Zi-Ling Zhu; Yu-Feng Feng; Fei-Ran Gong; De-Pei Wu

doi:10.1007/s12013-015-0655-z

Comparison of SPE, IFE, and FLC in Monitoring Patients with Multiple Myeloma After Autologous Stem Cell Transplantation

Cell Biochem Biophys. 2015 Dec;73(3):643-7. doi: 10.1007/s12013-015-0655-z.

Authors

Wei Li^{1

2}, Jia-Zi Zhou^{3

4

5

6}, Hui-Rong Chang^{3

4

5

6}, Li-Jun Dai^{3

4

5

6}, Zi-Ling Zhu^{3

4

5

6}, Yu-Feng Feng^{3

4

5

6}, Fei-Ran Gong^{7

8

9

10}, De-Pei Wu^{11

12

13

14}

Affiliations

¹ Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, China.
² PREMED Key Laboratory for Precision Medicine, Soochow University, Suzhou, China.
³ Department of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China.
⁴ Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.
⁵ Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
⁶ Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China.
⁷ Department of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China. [email protected].
⁸ Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China. [email protected].
⁹ Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China. [email protected].
¹⁰ Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China. [email protected].
¹¹ Department of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China. [email protected].
¹² Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China. [email protected].
¹³ Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China. [email protected].
¹⁴ Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China. [email protected].

PMID: 27259306
DOI: 10.1007/s12013-015-0655-z

Abstract

Conventionally, serum protein electrophoresis (SPE) and serum immunofixation electrophoresis (IFE) are used as primary methods to diagnose and monitor multiple myeloma (MM). Recently, serum-free light chain (FLC) assay has been incorporated into hematological screening programs for myeloma. The purpose of this study is to compare the performance of the three methods in monitoring MM patients after autologous stem cell transplantation (ASCT). SPE, serum IFE and serum FLC assay were performed on 38 MM patients who underwent ASCT. In total, four patients had unexpected protein bands (UPBs) and 13 patients had relapsed after ASCT. Our results indicate that IFE is more sensitive than SPE and FLC assay in detection of UPBs and relapse. The results of IFE may provide useful information in advance of patient relapse.

Keywords: ASCT; Multiple myeloma; SPE; Serum FLC ratio; Serum IFE.

Publication types

Comparative Study

MeSH terms

Blood Protein Electrophoresis / methods*
Blood Proteins / analysis*
Hematologic Tests / methods*
Humans
Immunoassay / methods
Multiple Myeloma / blood*
Multiple Myeloma / surgery
Multiple Myeloma / therapy
Sensitivity and Specificity
Stem Cell Transplantation / adverse effects*
Transplantation, Autologous / adverse effects*

Substances

Blood Proteins