Relationship between HHV8 infection markers and insulin sensitivity in ketosis-prone diabetes

J-L Nguewa; E Lontchi-Yimagou; F Agbelika; M AitDjoudi; P Boudou; S Choukem; E Sobngwi; J-F Gautier

doi:10.1016/j.diabet.2016.05.004

Relationship between HHV8 infection markers and insulin sensitivity in ketosis-prone diabetes

Diabetes Metab. 2017 Feb;43(1):79-82. doi: 10.1016/j.diabet.2016.05.004. Epub 2016 Jun 1.

Authors

J-L Nguewa¹, E Lontchi-Yimagou², F Agbelika³, M AitDjoudi⁴, P Boudou⁵, S Choukem⁶, E Sobngwi⁷, J-F Gautier⁸

Affiliations

¹ Department of Endocrinology and Diabetes, Lariboisière Hospital, University Paris-Diderot Paris-7, 2, rue Ambroise-Paré, 75010 Paris, France; Inserm UMRS 1138, Cordeliers Research Centre, 15, rue de l'École-de-Medicine, 75006 Paris, France. Electronic address: [email protected].
² Centre for biotechnology, Yaoundé University, Yaoundé, Cameroon.
³ Department of microbiology, Saint-Louis Hospital, University Paris-Diderot Paris-7, 1, avenue Claude-Vellefaux, 75010 Paris, France.
⁴ Department of Endocrinology and Diabetes, Lariboisière Hospital, University Paris-Diderot Paris-7, 2, rue Ambroise-Paré, 75010 Paris, France.
⁵ Department of biochemistry, Saint-Louis Hospital, University Paris-Diderot Paris-7, 1, avenue Claude-Vellefaux, 75010 Paris, France.
⁶ Department of internal medecine and Endocrinology, Douala General Hospital, PO Box 4856, Douala, Cameroon; Faculty of Medical Sciences, Buea University, Buea, Cameroon.
⁷ Centre for biotechnology, Yaoundé University, Yaoundé, Cameroon; National Centre for Obesity, Diabetes, Endocrinology and metabolic diseases, Yaoundé Central Hospital, PO Box 87, Yaoundé, Cameroon.
⁸ Department of Endocrinology and Diabetes, Lariboisière Hospital, University Paris-Diderot Paris-7, 2, rue Ambroise-Paré, 75010 Paris, France; Inserm UMRS 1138, Cordeliers Research Centre, 15, rue de l'École-de-Medicine, 75006 Paris, France; Clinical Investigation Centre, Inserm-CIC 9504, Saint-Louis Hospital, 75010 Paris, France.

PMID: 27262367
DOI: 10.1016/j.diabet.2016.05.004

Abstract

Background and objectives: Peripheral tissue resistance to insulin action is a characteristic of type 2 diabetes mellitus (T2DM). It has also been reported that some chronic viral infections can contribute to insulin resistance. Human herpesvirus (HHV)-8 infection has been detected in T2DM patients in previous studies. Our study investigated whether the presence of the virus is associated with insulin resistance in patients with ketosis-prone type 2 diabetes (KPD), as reported with other viruses.

Research design and methods: A total of 11 insulin-free KPD patients positive (+) and seven patients who were negative (-) for HHV-8 infection were recruited; the latter had KPD that was well controlled (HbA_1c=6.2±0.7%). A two-step euglycaemic-hyperinsulinaemic clamp test coupled with deuterated [6,6-2H2]glucose was used to assess insulin sensitivity, non-esterified fatty acid (NEFA) suppression and endogenous glucose production.

Results: In KPD patients, whether HHV-8+ or HHV-8-, there were no differences in NEFA release, endogenous glucose production or insulin sensitivity (M value).

Conclusion: Asymptomatic HHV-8 infection does not appear to be associated with decreased insulin sensitivity in diabetic patients. These results should now be confirmed in a larger sample population.

Keywords: Human herpesvirus 8; Insulin resistance; Ketosis-prone type 2 diabetes; Type 2 diabetes.

MeSH terms

Adult
Diabetes Mellitus, Type 2* / epidemiology
Diabetes Mellitus, Type 2* / virology
Diabetic Ketoacidosis* / epidemiology
Diabetic Ketoacidosis* / virology
Female
Glucose Clamp Technique
Herpesviridae Infections* / complications
Herpesviridae Infections* / epidemiology
Herpesvirus 8, Human*
Humans
Insulin Resistance*
Male
Middle Aged