Prevalence of vector-borne pathogens in dogs from Haiti

Vet Parasitol. 2016 Jul 15:224:7-12. doi: 10.1016/j.vetpar.2016.04.017. Epub 2016 Apr 19.

Abstract

Canine vector-borne pathogens are common on some Caribbean islands, but survey data in Haiti are lacking. To determine the prevalence of selected vector-borne pathogens in dogs from Haiti, we tested blood samples collected from 210 owned dogs, 28 (13.3%) of which were infested with Rhipicephalus sanguineus ticks at the time of blood collection. No other tick species were identified on these dogs. A commercially available ELISA identified antibodies to Ehrlichia spp. in 69 (32.9%), antibodies to Anaplasma spp. in 37 (17.6%), and antigen of Dirofilaria immitis in 55 (26.2%); antibodies to Borrelia burgdorferi were not detected in any sample. Molecular assays of whole blood from 207 of the dogs confirmed infection with Ehrlichia canis (15; 7.2%), Anaplasma platys (13; 6.3%), D. immitis (46; 22.2%), Wolbachia spp. (45; 21.7%), Babesia vogeli (16; 7.7%), and Hepatozoon canis (40; 19.3%), but Anaplasma phagocytophilum, Babesia canis, Babesia rossi, Babesia gibsoni, Ehrlichia chaffeensis, Ehrlichia ewingii, or Hepatozoon americanum were not detected. Co-infection with two or more vector-borne pathogens was detected by serology in 42 (20.0%) dogs and by molecular assays in 22 (10.6%) dogs; one dog was co-infected with B. vogeli and E. canis as detected by PCR with D. immitis detected by serology (antigen). Overall, evidence of past or current infection with at least one vector-borne pathogen was identified in 142/210 (67.6%) dogs in this study, underscoring the common nature of these pathogens, some of which are zoonotic, in Haiti.

Keywords: Anaplasma platys; Babesia vogeli; Dirofilaria immitis; Ehrlichia canis; Hepatozoon canis; Rhipicephalus sanguineus.

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood
  • Antibodies, Helminth / blood
  • Bacteria / genetics
  • Coinfection / epidemiology
  • Coinfection / veterinary
  • Dog Diseases / epidemiology*
  • Dog Diseases / microbiology
  • Dog Diseases / parasitology
  • Dogs
  • Enzyme-Linked Immunosorbent Assay
  • Haiti / epidemiology
  • Parasites / genetics
  • Polymerase Chain Reaction
  • Prevalence
  • Tick-Borne Diseases / epidemiology
  • Tick-Borne Diseases / microbiology
  • Tick-Borne Diseases / parasitology
  • Tick-Borne Diseases / veterinary*
  • Ticks / microbiology
  • Ticks / parasitology

Substances

  • Antibodies, Bacterial
  • Antibodies, Helminth