Identification and typing of the emerging pathogen Candida auris by matrix-assisted laser desorption ionisation time of flight mass spectrometry

Mycoses. 2016 Aug;59(8):535-8. doi: 10.1111/myc.12519. Epub 2016 Jun 13.

Abstract

Candida auris is an emerging antifungal resistant yeast species causing nosocomial and invasive infections, emphasising the need of improved diagnostics and epidemiological typing methods. We show that MALDI-TOF VITEK-MS followed by amplified length polymorphisms allows for accurate species identification and subsequent epidemiological characterisation of strains encountered during potential outbreaks.

Keywords: Candida auris; MS VITEK; amplified fragment length polymorphism; matrix-assisted laser desorption ionisation-time of flight mass spectrometry.

MeSH terms

  • Antifungal Agents / pharmacology
  • Candida / classification*
  • Candida / drug effects
  • Candida / isolation & purification*
  • Candida / pathogenicity
  • Candidiasis / diagnosis
  • Candidiasis / microbiology*
  • Disease Outbreaks
  • Humans
  • Microbial Sensitivity Tests
  • Polymorphism, Genetic
  • Sequence Analysis, DNA
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods

Substances

  • Antifungal Agents