Chemically Engineered Nanoparticle-Protein Interface for Real-Time Cellular Oxidative Stress Monitoring

Ying Jiang; Ming Wang; Joseph Hardie; Gulen Yesilbag Tonga; Moumita Ray; Qiaobing Xu; Vincent M Rotello

doi:10.1002/smll.201600986

Chemically Engineered Nanoparticle-Protein Interface for Real-Time Cellular Oxidative Stress Monitoring

Small. 2016 Jul;12(28):3775-9. doi: 10.1002/smll.201600986. Epub 2016 Jun 13.

Authors

Ying Jiang¹, Ming Wang², Joseph Hardie¹, Gulen Yesilbag Tonga¹, Moumita Ray¹, Qiaobing Xu², Vincent M Rotello¹

Affiliations

¹ Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA, 01003, USA.
² Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA, 02115, USA.

Abstract

A co-engineered nanoparticle/protein peroxide detector is created. This system features a gold nanoparticle functionalized with a galactose headgroup (AuNP-Gal) that reacts covalently with a boronate-modified green fluorescent protein (PB-GFP). Boronate acid-saccharide complexation between PB-GFP and AuNP-Gal affords a highly stable assembly. This complex is disrupted by peroxide, allowing quantitative and selective monitoring of hydrogen peroxide production in real time.

Keywords: cellular oxidative stress; chemically modified protein; gold nanoparticles; green fluorescent proteins; hydrogen peroxide.

MeSH terms

Biosensing Techniques / methods*
Galactose / chemistry
Gold / chemistry*
Green Fluorescent Proteins / chemistry
Hydrogen Peroxide / chemistry*
Metal Nanoparticles / chemistry*
Oxidative Stress / physiology*

Substances

Green Fluorescent Proteins
Gold
Hydrogen Peroxide
Galactose

Abstract

MeSH terms

Substances

Grants and funding