Oryzacystatins Exhibit Growth-inhibitory and Lethal Effects on Different Species of Bean Insect Pests, Callosobruchus chinensis (Coleoptera) and Riptortus clavatus (Hemiptera)

M Kuroda; M Ishimoto; K Suzuki; H Kondo; K Abe; K Kitamura; S Arai

doi:10.1271/bbb.60.209

Oryzacystatins Exhibit Growth-inhibitory and Lethal Effects on Different Species of Bean Insect Pests, Callosobruchus chinensis (Coleoptera) and Riptortus clavatus (Hemiptera)

Biosci Biotechnol Biochem. 1996 Jan;60(2):209-12. doi: 10.1271/bbb.60.209.

Authors

M Kuroda¹, M Ishimoto^{1

2}, K Suzuki^{1

3}, H Kondo^{1

4}, K Abe¹, K Kitamura^{1

2

3}, S Arai¹

Affiliations

¹ a Division of Agriculture and Agricultural Life Sciences, Department of Applied Biological Chemistry , The University of Tokyo , Bunkyo-ku, Tokyo 113 , Japan.
² b National Agriculture Research Center , Tsukuba, Ibaraki 305 , Japan.
³ c Cooperated Graduate School, The University of Tsukuha , Tsukuba, Ibaraki 305 , Japan.
⁴ d Suntory Co., Ltd. , Mishima-gun, Osaka 618 , Japan.

PMID: 27299395
DOI: 10.1271/bbb.60.209

Abstract

Oryzacystatins I and II, cysteine proteinase inhibitors in rice seeds, caused growth retardation of different species of bean insect pests, Callosobruchus chinensis (Coleoptera) and Riptortus clavatus (Hemiptera), when added to their diets at concentrations of 0.3-0.5% (w/w). At concentrations of up to 1%, almost all insects died. Our results suggest the usefulness of cystatin for insect pest control and also the critical role of cysteine proteinase in the digestive events of insects.

Keywords: cysteine proteinase; cysteine proteinase inhibitor; insect pest; oryzacystatin; rice seed.