Quantitative methods for studying hemostasis in zebrafish larvae

Methods Cell Biol. 2016:134:377-89. doi: 10.1016/bs.mcb.2016.01.003. Epub 2016 Feb 28.

Abstract

Hemostasis is a coordinated system through which blood is prevented from exiting a closed circulatory system. We have taken advantage of the zebrafish, an emerging model for the study of blood coagulation, and describe three techniques for quantitative analysis of primary and secondary hemostasis. Collectively, these three techniques comprise a toolset to aid in our understanding of hemostasis and pathological clotting.

Keywords: Clotting; Coagulation; Fibrinogen; Hematology; Hemostasis; Thrombosis; Zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Blood Coagulation / genetics*
  • Hemostasis / genetics*
  • Humans
  • Larva / genetics
  • Larva / physiology
  • Thrombosis / genetics*
  • Thrombosis / physiopathology
  • Zebrafish / genetics
  • Zebrafish / physiology