Use of New Techniques in Addition to IHC Applied to the Diagnosis of Melanocytic Lesions, With Emphasis on CGH, FISH, and Mass Spectrometry

P Nagarajan; M T Tetzlaff; J L Curry; V G Prieto

doi:10.1016/j.ad.2016.05.005

Use of New Techniques in Addition to IHC Applied to the Diagnosis of Melanocytic Lesions, With Emphasis on CGH, FISH, and Mass Spectrometry

Actas Dermosifiliogr. 2017 Jan-Feb;108(1):17-30. doi: 10.1016/j.ad.2016.05.005. Epub 2016 Jun 22.

[Article in English, Spanish]

Authors

P Nagarajan¹, M T Tetzlaff¹, J L Curry¹, V G Prieto²

Affiliations

¹ Department of Pathology, Unit 85, University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States.
² Department of Pathology, Unit 85, University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States. Electronic address: [email protected].

PMID: 27344067
DOI: 10.1016/j.ad.2016.05.005

Abstract

Melanoma remains one of the most aggressive forms of cutaneous malignancies. While its diagnosis based on histologic parameters is usually straight forward in most cases, distinguishing a melanoma from a melanocytic nevus can be challenging in some instances, especially when there are overlapping clinical and histopathologic features. Occasionally, melanomas can histologically mimic other tumors and even demonstration of melanocytic origin can be challenging. Thus, several ancillary tests may be employed to arrive at the correct diagnosis. The objective of this review is to summarize these tests, including the well-established and commonly used ones such as immunohistochemistry, with specific emphasis on emerging techniques such as comparative genomic hybridization, fluorescence in situ hybridization and imaging mass spectrometry.

Keywords: Comparative genomic hybridization; Diagnosis; Diagnóstico; Espectometría de masas; Fluorescence in situ hybridization; Hibridación genómica comparativa; Hibridación in situ con fluorescencia; Immunohistochemistry; Inmunohistoquímica; Mass spectrometry; Melanoma.

Publication types

Review

MeSH terms

Antigens, Neoplasm / analysis
Antigens, Neoplasm / genetics
Biomarkers, Tumor / analysis*
Biomarkers, Tumor / genetics
Cell Differentiation
Comparative Genomic Hybridization*
Dermatology / methods*
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence*
Mass Spectrometry*
Melanocytes / chemistry
Melanocytes / pathology
Melanoma / chemistry
Melanoma / diagnosis*
Melanoma / genetics
Melanoma / pathology
Neoplasm Proteins / analysis
Neoplasm Proteins / genetics
Nevus, Pigmented / chemistry
Nevus, Pigmented / diagnosis*
Nevus, Pigmented / genetics
Nevus, Pigmented / pathology
Prognosis
Skin Neoplasms / chemistry
Skin Neoplasms / diagnosis*
Skin Neoplasms / genetics
Skin Neoplasms / pathology
Staining and Labeling

Substances

Antigens, Neoplasm
Biomarkers, Tumor
Neoplasm Proteins