Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1

Charlotte Deloizy; Edwige Bouguyon; Even Fossum; Peter Sebo; Radim Osicka; Angélique Bole; Michel Pierres; Stéphane Biacchesi; Marc Dalod; Bjarne Bogen; Nicolas Bertho; Isabelle Schwartz-Cornil

doi:10.1016/j.dci.2016.06.015

Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1

Dev Comp Immunol. 2016 Dec:65:31-40. doi: 10.1016/j.dci.2016.06.015. Epub 2016 Jun 21.

Authors

Affiliations

¹ VIM-INRA-Université Paris-Saclay, Domaine de Vilvert, 78350, Jouy-en-Josas, France.
² K.G. Jebsen Center for Research on Influenza Vaccines, University of Oslo and Oslo University Hospital, 0027, Oslo, Norway.
³ Institute of Microbiology of the Czech Academy of Sciences, v.v.i., 142 20, Prague, Czech Republic.
⁴ MI-mAbs, Parc Scientifique et Technologique de Luminy, Case 906, F13288, Marseille Cedex 9, France.
⁵ Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université UM2, Inserm, U1104, CNRS UMR7280, 13288, Marseille, France.
⁶ K.G. Jebsen Center for Research on Influenza Vaccines, University of Oslo and Oslo University Hospital, 0027, Oslo, Norway; Center for Immune Regulation, Institute of Immunology, University of Oslo and Oslo University Hospital Rikshospitalet, 0424, Oslo, Norway.
⁷ VIM-INRA-Université Paris-Saclay, Domaine de Vilvert, 78350, Jouy-en-Josas, France. Electronic address: [email protected].

PMID: 27345169
DOI: 10.1016/j.dci.2016.06.015

Abstract

Pig is a domestic species of major importance in the agro-economy and in biomedical research. Mononuclear phagocytes (MNP) are organized in subsets with specialized roles in the orchestration of the immune response and new tools are awaited to improve MNP subset identification in the pig. We cloned pig CD11c cDNA and generated a monoclonal antibody to pig CD11c which showed a pattern of expression by blood and skin MNP subsets similar to humans. We also developed a porcine XCL1-mCherry dimer which specifically reacted with the XCR1-expressing dendritic cell subset of the type 1 lineage in blood and skin. These original reagents will allow the efficient identification of pig MNP subsets to study their role in physiological and pathological processes and also to target these cells in novel intervention and vaccine strategies for veterinary applications and preclinical evaluations.

Keywords: CD11c; Dendritic cells; Mononuclear phagocytes; Pig model; XCR1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal / metabolism
CD11c Antigen / immunology
CD11c Antigen / metabolism*
Cloning, Molecular
Dendritic Cells / physiology*
Disease Models, Animal
Humans
Immunologic Tests / methods
Mononuclear Phagocyte System*
Phagocytes / immunology*
Receptors, G-Protein-Coupled / immunology
Receptors, G-Protein-Coupled / metabolism*
Skin / metabolism*
Swine / immunology*
Veterinary Medicine

Substances

Antibodies, Monoclonal
CD11c Antigen
Receptors, G-Protein-Coupled