Human embryonic stem cells derived from abnormal blastocyst donated by glucose-6-phosphate dehydrogenase deficiency patient

Hao Zhou; Xiaoying Zhou; Qingqing Yang; Long Li; Juan Du; Guangxiu Lu; Ge Lin; Qi Ouyang

doi:10.1016/j.scr.2015.12.001

Human embryonic stem cells derived from abnormal blastocyst donated by glucose-6-phosphate dehydrogenase deficiency patient

Stem Cell Res. 2016 Jan;16(1):59-62. doi: 10.1016/j.scr.2015.12.001. Epub 2015 Dec 8.

Authors

Hao Zhou¹, Xiaoying Zhou², Qingqing Yang¹, Long Li¹, Juan Du³, Guangxiu Lu⁴, Ge Lin⁵, Qi Ouyang⁶

Affiliations

¹ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
² National Engineering and Research Center of Human Stem Cell, Changsha, China.
³ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
⁴ National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
⁵ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
⁶ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China. Electronic address: [email protected].

PMID: 27345785
DOI: 10.1016/j.scr.2015.12.001

Abstract

A human embryonic stem cell (hESC) line was derived from abnormal embryo donated by Glucose-6-phosphate dehydrogenase (G6PD) deficiency patient. Sequencing analysis confirmed that the hESC line possessed the mutant contributing to abnormal expression of G6PD. Further characteristic analysis demonstrated that the favism hESC line maintained stable and normal karyotype, expressed pluripotent markers and had the capacity of generating the derivatives from all three germ layers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaline Phosphatase / metabolism
Animals
Base Sequence
Blastocyst / pathology*
Cell Culture Techniques / methods*
Cell Differentiation
Cell Line
Glucosephosphate Dehydrogenase Deficiency / pathology*
Human Embryonic Stem Cells / cytology*
Humans
Karyotyping
Mice, SCID
Reproducibility of Results
Tissue Donors*

Substances

Alkaline Phosphatase