Derivation of the human embryonic stem cell line RCe006-A (RC-2)

Stem Cell Res. 2016 Mar;16(2):452-5. doi: 10.1016/j.scr.2016.02.029. Epub 2016 Feb 16.

Abstract

The human embryonic stem cell line RCe006-A (RC-2) was derived from a frozen and thawed blastocyst voluntarily donated as surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line exhibits expression of expected pluripotency markers and in vitro differentiation potential to three germinal lineage representative cell populations. It has a male trisomy 12 karyotype (47XY, +12). Microsatellite DNA marker identity and HLA and blood group typing data are available.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blastocyst / cytology*
  • Cell Differentiation
  • Cells, Cultured
  • Cellular Reprogramming
  • Chromosomes, Human, Pair 12
  • Comparative Genomic Hybridization
  • Down Syndrome / metabolism
  • Down Syndrome / pathology
  • Embryoid Bodies / cytology
  • Genotype
  • Histocompatibility Testing
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Karyotype
  • Male
  • Microscopy, Fluorescence
  • Polymerase Chain Reaction
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Trisomy

Substances

  • Transcription Factors