Derivation of the human embryonic stem cell line RCM1

Stem Cell Res. 2016 Mar;16(2):476-80. doi: 10.1016/j.scr.2015.12.020. Epub 2015 Dec 31.

Abstract

The human embryonic stem cell line RCM-1 was derived from a failed to fertilise egg undergoing parthenogenetic stimulation. The cell line shows normal pluripotency marker expression and differentiation to three germ layers in vitro and in vivo. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data is available.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Cellular Reprogramming
  • Comparative Genomic Hybridization
  • Embryoid Bodies / cytology
  • Female
  • Flow Cytometry
  • Histocompatibility Testing
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Human Embryonic Stem Cells / transplantation
  • Humans
  • Karyotype
  • Mice
  • Mice, SCID
  • Microsatellite Repeats / genetics
  • Microscopy, Fluorescence
  • Ovum / cytology*
  • Teratoma / metabolism
  • Teratoma / pathology
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transplantation, Heterologous

Substances

  • Transcription Factors