Derivation of the human embryonic stem cell line RCe011-A (RC-7)

Stem Cell Res. 2016 Mar;16(2):485-8. doi: 10.1016/j.scr.2016.02.036. Epub 2016 Feb 23.

Abstract

The human embryonic stem cell line RCe011-A (RC-7) was derived from a failed to fertilise oocyte voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XY male karyotype and microsatellite PCR identity, HLA and blood group typing data are available.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Cell Differentiation
  • Cells, Cultured
  • Embryo, Mammalian / cytology*
  • Embryoid Bodies / cytology
  • Genotype
  • Histocompatibility Testing
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Karyotype
  • Lewis X Antigen / metabolism
  • Male
  • Microsatellite Repeats / genetics
  • Microscopy, Fluorescence
  • Octamer Transcription Factor-3 / metabolism
  • Polymerase Chain Reaction

Substances

  • Lewis X Antigen
  • Octamer Transcription Factor-3
  • POU5F1 protein, human