Derivation of the human embryonic stem cell line RCe014-A (RC-10)

Stem Cell Res. 2016 Mar;16(2):537-40. doi: 10.1016/j.scr.2016.02.034. Epub 2016 Feb 23.

Abstract

The human embryonic stem cell line RCe014-A (RC-10) was derived from a fresh oocyte voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a mixed 46XY and 47XY +12 male karyotype and microsatellite PCR identity, HLA and blood group typing data is available.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation
  • Cells, Cultured
  • Chromosomes, Human, Pair 12
  • Embryo, Mammalian / cytology
  • Embryoid Bodies / cytology
  • Genotype
  • Histocompatibility Testing
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Immunohistochemistry
  • Karyotype
  • Male
  • Microsatellite Repeats / genetics
  • Microscopy, Fluorescence
  • Oocytes / cytology
  • Transcription Factors / metabolism
  • Trisomy

Substances

  • Transcription Factors