Generation of iPSC from cardiac and tail-tip fibroblasts derived from a second heart field reporter mouse

Stem Cell Res. 2016 May;16(3):617-21. doi: 10.1016/j.scr.2016.03.006. Epub 2016 Mar 19.

Abstract

Mef2c Anterior Heart Field (AHF) enhancer is activated during embryonic heart development and it is expressed in multipotent cardiovascular progenitors (CVP) giving rise to endothelial and myocardial components of the outflow tract, right ventricle and ventricular septum. Here we have generated iPSC from transgenic Mef2c-AHF-Cre x Ai6(RCLZsGreen) mice. These iPSC will provide a novel tool to investigate the AHF-CVP and their cell progeny.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cellular Reprogramming
  • Embryoid Bodies / cytology
  • Embryoid Bodies / metabolism
  • Fibroblasts / cytology*
  • Genotype
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotype
  • MEF2 Transcription Factors / genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Myocardium / cytology*
  • Teratoma / metabolism
  • Teratoma / pathology
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • MEF2 Transcription Factors
  • Mef2c protein, mouse
  • Transcription Factors