Derivation of the clinical grade human embryonic stem cell line RCe018-A (RC-14)

Stem Cell Res. 2016 May;16(3):761-5. doi: 10.1016/j.scr.2016.04.004. Epub 2016 Apr 13.

Abstract

The human embryonic stem cell line RCe018-A (RC-14) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a blastocyst stage embryo voluntarily donated as unsuitable or surplus to fertility requirements following informed consent. RCe018-A (RC-14) shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a male karyotype with an extra copy of chromosome 8 (47XY, +8). Microsatellite PCR identity, HLA and blood group typing data are available.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blastocyst / cytology*
  • Blood Group Antigens / metabolism
  • Cell Differentiation
  • Cells, Cultured
  • Cellular Reprogramming
  • Chromosomes, Human, Pair 8
  • Genotype
  • Histocompatibility Testing
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Karyotype
  • Male
  • Microsatellite Repeats / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Trisomy

Substances

  • Blood Group Antigens
  • Transcription Factors