Evaluation and validity of a polymerase chain reaction-based open reading frame typing method to dissect the molecular epidemiology for Acinetobacter baumannii in an epidemiologic study of a hospital outbreak

Yuji Fujikura; Atsushi Yuki; Takaaki Hamamoto; Sadahiro Ichimura; Akihiko Kawana; Kiyofumi Ohkusu; Tetsuya Matsumoto

doi:10.1016/j.ajic.2016.03.059

Evaluation and validity of a polymerase chain reaction-based open reading frame typing method to dissect the molecular epidemiology for Acinetobacter baumannii in an epidemiologic study of a hospital outbreak

Am J Infect Control. 2016 Nov 1;44(11):e275-e278. doi: 10.1016/j.ajic.2016.03.059. Epub 2016 Jun 24.

Authors

Yuji Fujikura¹, Atsushi Yuki², Takaaki Hamamoto², Sadahiro Ichimura³, Akihiko Kawana⁴, Kiyofumi Ohkusu⁵, Tetsuya Matsumoto⁵

Affiliations

¹ Department of Microbiology, Tokyo Medical University, Tokyo, Japan; Division of Infectious Diseases and Respiratory Medicine, National Defense Medical College, Saitama, Japan. Electronic address: [email protected].
² Department of Clinical Laboratory, National Defense Medical College Hospital, Saitama, Japan.
³ Department of Bacteriology, BML, Inc., Saitama, Japan.
⁴ Division of Infectious Diseases and Respiratory Medicine, National Defense Medical College, Saitama, Japan.
⁵ Department of Microbiology, Tokyo Medical University, Tokyo, Japan.

PMID: 27350114
DOI: 10.1016/j.ajic.2016.03.059

Abstract

Acinetobacter baumannii is regarded as one of the most important pathogens in hospital outbreaks. To obtain an efficient and simple epidemiologic method of surveillance during outbreaks, we assessed the applicability of the polymerase chain reaction-based open reading frames typing (POT) method and compared it with pulsed-field gel electrophoresis. The POT method was found to have sufficient discriminatory power to identify the strains and would be widely applicable to epidemiologic surveillance during hospital outbreaks.

Keywords: Acinetobacter baumannii; PCR-based open reading frames typing (POT) method; epidemiology; outbreak; pulsed-field gel electrophoresis.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Acinetobacter Infections / epidemiology*
Acinetobacter Infections / microbiology
Acinetobacter baumannii / classification*
Acinetobacter baumannii / genetics*
Acinetobacter baumannii / isolation & purification
Cross Infection / epidemiology*
Cross Infection / microbiology
Disease Outbreaks*
Electrophoresis, Gel, Pulsed-Field
Humans
Molecular Epidemiology / methods
Molecular Typing / methods*
Open Reading Frames
Polymerase Chain Reaction / methods*