Abstract
Axl encodes the tyrosine-protein kinase receptor, participating in the proliferation and migration of many cells. This study examined the role of Axl in functions of endothelial progenitor cells (EPCs). Axl was detected by RT-PCR and Western blotting in both placentas and EPCs from normal pregnancy and preeclampsia patients. The Axl inhibitor, BMS777-607, was used to inhibit the Axl signalling pathway in EPCs. Cell proliferation, differentiation, migration and adhesion were measured by CCK-8 assay, cell differentiation assay, Transwell assay, and cell adhesion assay, respectively. Results showed the expression levels of Axl mRNA and protein were significantly higher in both placentas and EPCs from preeclampsia patients than from normal pregnancy (P<0.05). After treatment with BMS777-607, proliferation, differentiation, migration and adhesion capability of EPCs were all significantly decreased. Our study suggests Axl may play a role in the function of EPCs, thereby involving in the pathogenesis of preeclampsia.
Keywords:
Axl; endothelial progenitor cells; preeclampsia.
MeSH terms
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Adult
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Aminopyridines / pharmacology
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Axl Receptor Tyrosine Kinase
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Blood Pressure
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Case-Control Studies
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Cell Adhesion / drug effects
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Cell Differentiation / drug effects
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Cell Movement / drug effects
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Cell Proliferation / drug effects
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Female
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Fetal Blood / cytology
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Fetal Blood / enzymology
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Gene Expression Regulation
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Gestational Age
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Human Umbilical Vein Endothelial Cells / drug effects
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Human Umbilical Vein Endothelial Cells / enzymology*
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Human Umbilical Vein Endothelial Cells / pathology
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Humans
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Placenta / metabolism
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Placenta / physiopathology
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Pre-Eclampsia / blood
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Pre-Eclampsia / genetics*
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Pre-Eclampsia / physiopathology
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Pregnancy
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Primary Cell Culture
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Protein Kinase Inhibitors / pharmacology
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Proto-Oncogene Proteins / antagonists & inhibitors
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins / metabolism
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Pyridones / pharmacology
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RNA, Messenger / antagonists & inhibitors
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RNA, Messenger / genetics*
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RNA, Messenger / metabolism
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Receptor Protein-Tyrosine Kinases / antagonists & inhibitors
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Receptor Protein-Tyrosine Kinases / genetics*
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Receptor Protein-Tyrosine Kinases / metabolism
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Stem Cells / drug effects
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Stem Cells / enzymology*
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Stem Cells / pathology
Substances
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Aminopyridines
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N-(4-(2-amino-3-chloropyridin-4-yloxy)-3-fluorophenyl)-4-ethoxy-1-(4-fluorophenyl)-2-oxo-1,2-dihydropyridine-3-carboxamide
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Protein Kinase Inhibitors
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Proto-Oncogene Proteins
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Pyridones
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RNA, Messenger
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Receptor Protein-Tyrosine Kinases
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Axl Receptor Tyrosine Kinase
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AXL protein, human