Abstract
Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Arabidopsis / classification
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Arabidopsis / genetics*
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Arabidopsis / growth & development
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Arabidopsis / metabolism
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Arabidopsis Proteins / genetics*
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Arabidopsis Proteins / metabolism
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Biological Transport
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Cell Wall / chemistry
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Cell Wall / metabolism
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Cloning, Molecular
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Gene Expression
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Genetic Vectors / chemistry
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Genetic Vectors / metabolism
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Glucans / biosynthesis
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Golgi Apparatus / chemistry
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Golgi Apparatus / metabolism*
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Guanosine Diphosphate Fucose / metabolism*
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Monosaccharide Transport Proteins / genetics*
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Monosaccharide Transport Proteins / metabolism
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Pectins / biosynthesis
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Phylogeny
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Plant Cells / chemistry
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Plant Cells / metabolism
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Proteolipids / chemistry
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Proteolipids / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Xylans / biosynthesis
Substances
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Arabidopsis Proteins
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Glucans
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Monosaccharide Transport Proteins
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Proteolipids
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Recombinant Proteins
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Xylans
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proteoliposomes
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rhamnogalacturonan II
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Guanosine Diphosphate Fucose
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xyloglucan
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Pectins