Hierarchical Fabrication of Engineered Vascularized Bone Biphasic Constructs via Dual 3D Bioprinting: Integrating Regional Bioactive Factors into Architectural Design

Adv Healthc Mater. 2016 Sep;5(17):2174-81. doi: 10.1002/adhm.201600505. Epub 2016 Jul 7.

Abstract

A biphasic artificial vascularized bone construct with regional bioactive factors is presented using dual 3D bioprinting platform technique, thereby forming a large functional bone grafts with organized vascular networks. Biocompatible mussel-inspired chemistry and "thiol-ene" click reaction are used to regionally immobilize bioactive factors during construct fabrication for modulating or improving cellular events.

Keywords: click reaction; dual 3D bioprinting; mussel-inspired chemistry; regional immobilization; vascularized complex tissue.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Bone and Bones* / blood supply
  • Bone and Bones* / cytology
  • Bone and Bones* / metabolism
  • Human Umbilical Vein Endothelial Cells / cytology
  • Human Umbilical Vein Endothelial Cells / metabolism*
  • Humans
  • Printing, Three-Dimensional*
  • Tissue Engineering*