Research Techniques Made Simple: Methodology and Clinical Applications of RNA Sequencing

Sarah K Whitley; William T Horne; Jay K Kolls

doi:10.1016/j.jid.2016.06.003

Research Techniques Made Simple: Methodology and Clinical Applications of RNA Sequencing

J Invest Dermatol. 2016 Aug;136(8):e77-e82. doi: 10.1016/j.jid.2016.06.003.

Authors

Sarah K Whitley¹, William T Horne², Jay K Kolls³

Affiliations

¹ Department of Dermatology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA. Electronic address: [email protected].
² Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA.
³ Department of Pediatrics, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA; Richard King Mellon Institute for Pediatric Research, Pittsburgh, Pennsylvania, USA.

PMID: 27450500
DOI: 10.1016/j.jid.2016.06.003

Abstract

RNA sequencing is a method of transcriptome profiling that utilizes next-generation sequencing technology. It offers several distinct advantages over hybridization-based approaches, most notably superior sensitivity and the capacity for de novo transcript discovery. This article describes RNA sequencing methodology, summarizes important technological advances and challenges, and discusses applications for this technique in the field of dermatology.

Publication types

Review

MeSH terms

Biopsy
Dermatology / methods*
Exons
Gene Expression Profiling / methods*
Gene Library
Genome, Human
High-Throughput Nucleotide Sequencing
Humans
Mutation
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis
Polymorphism, Single Nucleotide
Protein Isoforms
Sequence Analysis, RNA / methods*
Skin / metabolism
Transcriptome

Substances

Protein Isoforms