Abstract
Heparin stimulates 2-3-fold, in a concentration-dependent manner, the synthesis of heparan sulfate secreted by cultured endothelial cells. The increase in synthetic rate takes place immediately after exposure of the cells to heparin, affects only heparan sulfate, and is specific for the endothelial cell. No stimulation by other glycosaminoglycans was observed. Analysis of the disaccharide products formed by the action of heparitinases reveals a higher degree of sulfation of the uronic acid residues in the heparan sulfate of cells exposed to heparin.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cattle
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Cells, Cultured
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Chemical Phenomena
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Chemistry
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Electrophoresis, Agar Gel
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Endothelium, Vascular / cytology*
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / metabolism
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Glycosaminoglycans / metabolism
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Heparin / analogs & derivatives*
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Heparin / metabolism
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Heparin / pharmacology*
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Heparitin Sulfate / analysis
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Heparitin Sulfate / metabolism
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Proteoglycans / metabolism*
Substances
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Glycosaminoglycans
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Proteoglycans
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heparin proteoglycan
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Heparin
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Heparitin Sulfate