Comparative study of CD4 and CD45RO T cells and CD20 B cells in cerebrospinal fluid of syphilitic meningitis and tuberculous meningitis patients

APMIS. 2016 Sep;124(9):764-9. doi: 10.1111/apm.12572. Epub 2016 Jul 28.

Abstract

This study was to investigate the differences of lymphocyte in the cerebrospinal fluid (CSF) of patients with syphilis meningitis (SM) and tuberculous meningitis (TBM) for new diagnostic insights. Totally, 79 cases of SM and 45 cases of TBM were enrolled. In the CSF, the CD4, CD45RO or CD20 positive lymphocytes were detected by immunohistochemistry. The proportion of CD4 T cells in the CSF lymphocytes in patients with SM was significantly higher than that in patients with TBM (p < 0.05). After medical therapy, there was a significantly decline trend of the CD4 T-cell proportion in both groups (p < 0.05). The proportion of CD45RO T cells in CSF lymphocytes of patients with SM was less than that of patients with TBM (p < 0.05). After medical therapy, the positive ratio of CD45RO T cells was increased in the CSF of both group patients (p < 0.05). The proportion of CD20B cells in the CSF lymphocytes was not obviously different between the two groups during every stage. In conclusion, there are strong differences of CD4 and CD45RO T-cell ratio, but not the CD20 B cells in the meningitis. CD4 and CD45RO T cells in CSF are a useful complement in differentially diagnosing SM and TBM; it contributes to further understand the pathogenesis and prognosis of SM and TBM.

Keywords: CD20 B cells; CD4 T cells; CD45RO T cells; syphilitic meningitis; tuberculous meningitis.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Antigens, CD20 / analysis
  • B-Lymphocytes / chemistry
  • B-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / chemistry
  • CD4-Positive T-Lymphocytes / immunology*
  • Cerebrospinal Fluid / cytology*
  • Diagnosis, Differential
  • Female
  • Humans
  • Immunohistochemistry
  • Immunophenotyping
  • Leukocyte Common Antigens / analysis
  • Lymphocyte Subsets / chemistry
  • Lymphocyte Subsets / immunology*
  • Male
  • Neurosyphilis / pathology*
  • Tuberculosis, Meningeal / pathology*

Substances

  • Antigens, CD20
  • Leukocyte Common Antigens

Supplementary concepts

  • Syphilitic aseptic meningitis