Characterization of p75 neurotrophin receptor expression in human dental pulp stem cells

Wenru Pan; Karlea L Kremer; Xenia Kaidonis; Victoria E Ludlow; Mary-Louise Rogers; Jianling Xie; Christopher G Proud; Simon A Koblar

doi:10.1016/j.ijdevneu.2016.07.007

Characterization of p75 neurotrophin receptor expression in human dental pulp stem cells

Int J Dev Neurosci. 2016 Oct:53:90-98. doi: 10.1016/j.ijdevneu.2016.07.007. Epub 2016 Jul 26.

Authors

Wenru Pan¹, Karlea L Kremer², Xenia Kaidonis², Victoria E Ludlow², Mary-Louise Rogers³, Jianling Xie⁴, Christopher G Proud⁵, Simon A Koblar⁶

Affiliations

¹ School of Medical Science, Australia; South Australian Health & Medical Research Institute, South Australia, Australia.
² School of Medicine, Australia; South Australian Health & Medical Research Institute, South Australia, Australia.
³ Human Physiology, Centre for Neuroscience, School of Medicine, Flinders University, Australia.
⁴ South Australian Health & Medical Research Institute, South Australia, Australia.
⁵ School of Biological Sciences, University of Adelaide, Australia; South Australian Health & Medical Research Institute, South Australia, Australia.
⁶ School of Medicine, Australia; South Australian Health & Medical Research Institute, South Australia, Australia. Electronic address: [email protected].

PMID: 27469433
DOI: 10.1016/j.ijdevneu.2016.07.007

Abstract

Human adult dental pulp stem cells (DPSC) are a heterogeneous stem cell population, which are able to differentiate down neural, chondrocyte, osteocyte and adipocyte lineages. We studied the expression pattern of p75 neurotrophin receptors (p75NTR), a marker of neural stem cells, within human DPSC populations from eight donors. p75NTR are expressed at low levels (<10%) in DPSC. Importantly, p75(+) DPSC represent higher expression levels of SOX1 (neural precursor cell marker), SOX2 (cell pluripotency marker) and nestin (neural stem cell marker) in comparison to p75(-) DPSC. Our results suggest that p75(+) hDPSC may denote a subpopulation with greater neurogenic potential.

Keywords: Dental pulp stem cells; Neural crest stem cells; SOX2; SOX9; p75 Neurotrophin receptor.

MeSH terms

Cell Differentiation / physiology
Cells, Cultured
Dental Pulp / cytology*
Flow Cytometry
Humans
Nerve Tissue Proteins / metabolism*
Receptors, Nerve Growth Factor / metabolism*
SOXB1 Transcription Factors / metabolism
Stem Cells / metabolism*

Substances

NGFR protein, human
Nerve Tissue Proteins
Receptors, Nerve Growth Factor
SOX1 protein, human
SOX2 protein, human
SOXB1 Transcription Factors