Serum cytokine profile contributes to discriminating M. pneumoniae pneumonia in children

Cytokine. 2016 Oct:86:73-78. doi: 10.1016/j.cyto.2016.07.018. Epub 2016 Jul 29.

Abstract

Background: To evaluate the role of serum cytokines in discriminating M. pneumoniae infection in children with community-acquired pneumonia (CAP).

Methods: A prospective observational study was conducted. 385 hospitalized patients with CAP had only M. pneumoniae (MP group) infection; 321 hospitalized patients with CAP had no M. pneumoniae and other specific pathogen (control group) infections. Serum interleukin-2 (IL-2), IL-4, IL-6, IL-10, tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) were detected by flow cytometry.

Results: In children younger than 5years, serum IL-6, TNF-α, and IFN-γ levels from MP group were significantly higher than those from control group. However in children 5-15years, serum IL-6, IL-10, and IFN-γ levels from MP group were significantly higher than those from control group. In the final multivariate logistic regression model for serum cytokine, moderately elevated IL-6, IL-10, and IFN-γ shows a higher prediction of development of M. pneumoniae pneumonia among CAP patients.

Conclusions: A specific cytokine pattern showed a higher prediction of M. pneumoniae pneumonia among CAP patients, further suggesting that serum cytokine pattern might be useful in differentiating infectious causative agents in children.

Keywords: Children; Community-acquired pneumonia; Cytokine; M. pneumoniae.

Publication types

  • Observational Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Child
  • Child, Preschool
  • Community-Acquired Infections / blood
  • Community-Acquired Infections / diagnosis*
  • Community-Acquired Infections / immunology*
  • Community-Acquired Infections / microbiology
  • Cytokines / blood*
  • Female
  • Flow Cytometry
  • Hospitalization
  • Humans
  • Infant
  • Interferon-gamma / blood
  • Interleukin-10 / blood
  • Interleukin-2 / blood
  • Interleukin-4 / blood
  • Interleukin-6 / blood
  • Male
  • Mycoplasma pneumoniae / immunology*
  • Mycoplasma pneumoniae / isolation & purification
  • Pneumonia, Mycoplasma / blood
  • Pneumonia, Mycoplasma / diagnosis*
  • Pneumonia, Mycoplasma / immunology*
  • Prospective Studies
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Cytokines
  • IL10 protein, human
  • IL4 protein, human
  • IL6 protein, human
  • Interleukin-2
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Interleukin-4
  • Interferon-gamma