The ease of use, robustness, cost-effectiveness, and posttranslational machinery make baculovirus expression system a popular choice for production of eukaryotic membrane proteins. This system can be readily adapted for high-throughput operations. This chapter outlines the techniques and procedures for cloning, transfection, small-scale production, and purification of membrane protein samples in a high-throughput manner.
Keywords: Bacmid; Baculovirus; Expression; High-throughput; Insect cell; Membrane protein; Nickel affinity chromatography; Purification; Sf9.