Protective efficacy of multiple vaccine platforms against Zika virus challenge in rhesus monkeys

Science. 2016 Sep 9;353(6304):1129-32. doi: 10.1126/science.aah6157. Epub 2016 Aug 4.

Abstract

Zika virus (ZIKV) is responsible for a major ongoing epidemic in the Americas and has been causally associated with fetal microcephaly. The development of a safe and effective ZIKV vaccine is therefore an urgent global health priority. Here we demonstrate that three different vaccine platforms protect against ZIKV challenge in rhesus monkeys. A purified inactivated virus vaccine induced ZIKV-specific neutralizing antibodies and completely protected monkeys against ZIKV strains from both Brazil and Puerto Rico. Purified immunoglobulin from vaccinated monkeys also conferred passive protection in adoptive transfer studies. A plasmid DNA vaccine and a single-shot recombinant rhesus adenovirus serotype 52 vector vaccine, both expressing ZIKV premembrane and envelope, also elicited neutralizing antibodies and completely protected monkeys against ZIKV challenge. These data support the rapid clinical development of ZIKV vaccines for humans.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae
  • Adoptive Transfer
  • Animals
  • Antibodies, Viral / biosynthesis
  • Antibodies, Viral / immunology
  • Brazil
  • Female
  • Genetic Vectors
  • Humans
  • Immunogenicity, Vaccine*
  • Immunoglobulins / immunology
  • Immunoglobulins / isolation & purification
  • Macaca mulatta
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Puerto Rico
  • Vaccines, DNA / administration & dosage
  • Vaccines, DNA / immunology*
  • Vaccines, Inactivated / administration & dosage
  • Vaccines, Inactivated / immunology
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology*
  • Zika Virus / immunology*
  • Zika Virus Infection / prevention & control*

Substances

  • Antibodies, Viral
  • Immunoglobulins
  • Vaccines, DNA
  • Vaccines, Inactivated
  • Viral Envelope Proteins
  • Viral Vaccines
  • prM protein, Flavivirus