Chromatin immunoprecipitation (ChIP) has been widely used for studying in vivo protein-DNA interactions for decades. ChIP is a powerful tool that is adaptable for studying epigenetic modifications at certain genomic loci or the genomic level. Given its utility in studying the epigenome and the many technical challenges, we present a detailed in-lab ChIP protocol primarily used for studying histone modifications in plants, but can be easily adapted for other chromatin targets in other species.
Keywords: Antibody; Chromatin immunoprecipitation; Cross-linking; Epigenetic modifications; Epigenome; Histone.