Differential Releases of Dopamine and Neuropeptide Y from Histamine-Stimulated PC12 Cells Detected by an Aptamer-Modified Nanowire Transistor

Subhasree Banerjee; Ying-Jhu Hsieh; Chia-Rung Liu; Nai-Hsing Yeh; Hui-Hsing Hung; Yew-Seng Lai; Ai-Chuan Chou; Yit-Tsong Chen; Chien-Yuan Pan

doi:10.1002/smll.201601370

Differential Releases of Dopamine and Neuropeptide Y from Histamine-Stimulated PC12 Cells Detected by an Aptamer-Modified Nanowire Transistor

Small. 2016 Oct;12(40):5524-5529. doi: 10.1002/smll.201601370. Epub 2016 Aug 23.

Authors

Subhasree Banerjee^{1

2

3}, Ying-Jhu Hsieh¹, Chia-Rung Liu¹, Nai-Hsing Yeh³, Hui-Hsing Hung³, Yew-Seng Lai¹, Ai-Chuan Chou³, Yit-Tsong Chen^{4

5}, Chien-Yuan Pan⁶

Affiliations

¹ Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan.
² Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei, 106, Taiwan.
³ Department of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan.
⁴ Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan. [email protected].
⁵ Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei, 106, Taiwan. [email protected].
⁶ Department of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan. [email protected].

PMID: 27551968
DOI: 10.1002/smll.201601370

Abstract

Silicon nanowire field-effect transistors modified with specific aptamers can directly detect the minute dopamine and neuropeptide Y released from cells. The binding of these molecules to the aptamers results in a conductance change of the transistor biosensor and illustrates the differential releasing mechanisms of these molecules stored in various vesicle pools.

Keywords: dopamine; histamine; large dense-core vesicles; neuropeptide Y; silicon nanowire field-effect transistors.

MeSH terms

Animals
Aptamers, Peptide / chemistry*
Dopamine / analysis*
Histamine / pharmacology*
Nanowires / chemistry*
Neuropeptide Y / analysis*
PC12 Cells
Rats
Silicon / chemistry
Transistors, Electronic*

Substances

Aptamers, Peptide
Neuropeptide Y
Histamine
Dopamine
Silicon