A human MIXL1 green fluorescent protein reporter embryonic stem cell line engineered using TALEN-based genome editing

Vera Alexeeva; Sunita L D'Souza; Christoph Schaniel

doi:10.1016/j.scr.2016.05.010

A human MIXL1 green fluorescent protein reporter embryonic stem cell line engineered using TALEN-based genome editing

Stem Cell Res. 2016 Jul;17(1):93-96. doi: 10.1016/j.scr.2016.05.010. Epub 2016 May 26.

Authors

Vera Alexeeva¹, Sunita L D'Souza², Christoph Schaniel³

Affiliations

¹ Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States; Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States.
² Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States; Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States; Experimental Therapeutics Institute, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States. Electronic address: sunita.d'[email protected].
³ Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States; Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States; Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States; Mount Sinai Institute for Systems Biomedicine, Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Place, Box 1496, New York, NY 10029, United States. Electronic address: [email protected].

PMID: 27558609
DOI: 10.1016/j.scr.2016.05.010

Abstract

We have generated a MIXL1-eGFP reporter human embryonic stem cell (hESC) line using TALEN-based genome engineering. This line accurately traces endogenous MIXL1 expression via an eGFP reporter to mesendodermal precursor cells. The utility of the MIXL1-eGFP reporter hESC line lies in the prospective isolation, lineage tracing, and developmental and mechanistic studies of MIXL1⁺ cell populations.