Bioactive diterpenoids from Trigonostemon chinensis: Structures, NO inhibitory activities, and interactions with iNOS

Bioorg Med Chem Lett. 2016 Oct 1;26(19):4785-4789. doi: 10.1016/j.bmcl.2016.08.026. Epub 2016 Aug 10.

Abstract

A phytochemical investigation to obtain new NO inhibitors led to the isolation of two new (1 and 2) and four known (3-6) diterpenoids from Trigonostemon chinensis. Their structures were elucidated on the basis of extensive 1D and 2D NMR spectroscopic data analyses, and the absolute configurations of new compounds were established by experimental and calculated ECD spectra. The inhibitory activities on lipopolysaccharide-induced NO production in murine microglial BV-2 cells of these diterpenoids were evaluated, and all of the compounds showed inhibitory effects. The interactions of bioactive compounds with iNOS protein were also studied by molecular docking.

Keywords: Diterpenoids; Molecular docking; NO inhibitory activities; Trigonostemon chinensis; iNOS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Diterpenes / chemistry*
  • Diterpenes / pharmacology*
  • Euphorbiaceae / chemistry*
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Nitric Oxide / antagonists & inhibitors*
  • Nitric Oxide Synthase Type II / antagonists & inhibitors*
  • Spectrometry, Mass, Electrospray Ionization

Substances

  • Diterpenes
  • Nitric Oxide
  • Nitric Oxide Synthase Type II