Structure and anticancer activity of sulfated O-polysaccharide from marine bacterium Cobetia litoralis KMM 3880(T)

Maxim S Kokoulin; Alexandra S Kuzmich; Anatoly I Kalinovsky; Svetlana V Tomshich; Lyudmila A Romanenko; Valery V Mikhailov; Nadezhda A Komandrova

doi:10.1016/j.carbpol.2016.08.036

Structure and anticancer activity of sulfated O-polysaccharide from marine bacterium Cobetia litoralis KMM 3880(T)

Carbohydr Polym. 2016 Dec 10:154:55-61. doi: 10.1016/j.carbpol.2016.08.036. Epub 2016 Aug 12.

Authors

Maxim S Kokoulin¹, Alexandra S Kuzmich², Anatoly I Kalinovsky², Svetlana V Tomshich², Lyudmila A Romanenko², Valery V Mikhailov³, Nadezhda A Komandrova²

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation. Electronic address: [email protected].
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation.
³ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, 690022, Vladivostok, Russian Federation; Far Eastern Federal University, Suhanova St., 8, 690950, Vladivostok, Russian Federation.

PMID: 27577896
DOI: 10.1016/j.carbpol.2016.08.036

Abstract

We presented the structure of the polysaccharide moiety and anticancer activity in vitro of the sulfated lipopolysaccharide isolated from the marine bacterium Cobetia litoralis KMM 3880(T). The structure of O-polysaccharide was investigated by chemical methods along with (1)H and (13)C NMR spectroscopy. The O-polysaccharide was built up of branched trisaccharide repeating units consist of D-glucose (D-Glcр), D-mannose (D-Manр) and sulfated 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo5S): →7-β-Kdoр4Ac5S-(2→4)-[β-d-Glcp-(1→2)-]-β-d-Manр6Ac-1→. We demonstrated that the lipopolysaccharide and О-deacetylated O-polysaccharide from Cobetia litoralis KMM 3880(T) inhibited a colony formation of human melanoma SK-MEL-28 and colorectal carcinoma HTC-116 cells.

Keywords: Anticancer activity; Cobetia litoralis; Lipopolysaccharide; Marine bacterium; O-polysaccharide; Sulfated 3-deoxy-d-manno-oct-2-ulosonic acid.

MeSH terms

Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology*
Aquatic Organisms
Carbohydrate Conformation
Carbohydrate Sequence
Cell Line, Tumor
Drug Screening Assays, Antitumor / methods
Gammaproteobacteria / chemistry*
HCT116 Cells
Humans
Magnetic Resonance Spectroscopy
Melanoma / drug therapy
Melanoma / pathology
Polysaccharides, Bacterial / chemistry*
Polysaccharides, Bacterial / pharmacology*
Spectroscopy, Fourier Transform Infrared
Sulfates / chemistry

Substances

Antineoplastic Agents
Polysaccharides, Bacterial
Sulfates