Changes in endothelial cell proliferation and vascular permeability after systemic lipopolysaccharide administration in the subfornical organ

J Neuroimmunol. 2016 Sep 15:298:132-7. doi: 10.1016/j.jneuroim.2016.06.011. Epub 2016 Jul 1.

Abstract

The subfornical organ (SFO) has highly permeable fenestrated vasculature and is a key site for immune-to-brain communications. Recently, we showed the occurrence of continuous angiogenesis in the SFO. In the present study, we found that systemic administration of bacterial lipopolysaccharide (LPS) reduced the vascular permeability and endothelial cell proliferation. In LPS-administered mice, the SFO vasculature showed a significant decrease in the immunoreactivity of plasmalemma vesicle associated protein-1, a marker of endothelial fenestral diaphragms. These data suggest that vasculature undergoes structural change to decrease vascular permeability in response to systemic LPS administration.

Keywords: Angiogenesis; Blood–brain barrier; Fenestrated endothelial cells; Inflammation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Bromodeoxyuridine / metabolism
  • Capillary Permeability / drug effects*
  • Carrier Proteins / metabolism
  • Cell Proliferation / drug effects*
  • Endothelial Cells / drug effects*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Lipopolysaccharides / toxicity*
  • Male
  • Membrane Proteins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Confocal
  • Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
  • Subfornical Organ / drug effects*
  • Subfornical Organ / pathology
  • Time Factors

Substances

  • Carrier Proteins
  • Lipopolysaccharides
  • Membrane Proteins
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Plvap protein, mouse
  • Green Fluorescent Proteins
  • Bromodeoxyuridine