FABP5 correlates with poor prognosis and promotes tumor cell growth and metastasis in cervical cancer

Tumour Biol. 2016 Nov;37(11):14873-14883. doi: 10.1007/s13277-016-5350-1. Epub 2016 Sep 19.

Abstract

Fatty acid-binding protein 5 (FABP5) was found in our previous study to be a potential biomarker for lymph node metastasis of cervical cancer. However, the roles of FABP5 in cervical cancer remain unclear. In the present study, FABP5 expression was found to be significantly upregulated in cervical cancer tissues, and high FABP5 expression was significantly correlated with lymph node metastasis, lymphovascular space invasion, the International Federation of Gynecology and Obstetrics (FIGO) stage, and tumor size. Moreover, FABP5 was an independent factor for poor prognosis in cervical cancer patients. Silencing of FABP5 inhibited cell proliferation, colony formation, cell migration, and invasion in vitro. Furthermore, FABP5 silencing significantly reduced tumor growth and lung metastases in a murine allograft model in vivo. In addition, FABP5 silencing decreased the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in vitro and in vivo. Collectively, these findings indicated that FABP5 plays an important role in the carcinogenesis and metastasis of cervical cancer, and FABP5 may be a novel predictor for prognostic assessment of cervical cancer patients.

Keywords: Cervical cancer; FABP5; Metastasis; Prognosis.

MeSH terms

  • Adult
  • Animals
  • Biomarkers, Tumor / genetics*
  • Carcinogenesis / genetics*
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics*
  • Fatty Acid-Binding Proteins / genetics*
  • Female
  • HeLa Cells
  • Humans
  • Lung Neoplasms / secondary
  • Lymphatic Metastasis
  • Matrix Metalloproteinase 2 / biosynthesis
  • Matrix Metalloproteinase 9 / biosynthesis
  • Mice
  • Mice, Nude
  • Neoplasm Invasiveness / genetics
  • Prognosis
  • RNA Interference
  • RNA, Small Interfering
  • Uterine Cervical Neoplasms / mortality
  • Uterine Cervical Neoplasms / pathology*

Substances

  • Biomarkers, Tumor
  • FABP5 protein, human
  • Fatty Acid-Binding Proteins
  • RNA, Small Interfering
  • MMP2 protein, human
  • Matrix Metalloproteinase 2
  • MMP9 protein, human
  • Matrix Metalloproteinase 9